Inhibition of transketolase by N-acetylimidazole
Kuimov, A.N.; Kovina, M.V.; Kochetov, G.A.
Biochemistry International 17(3): 517-521
1988
ISSN/ISBN: 0158-5231 PMID: 3060120 Document Number: 317598
Transketolase from baker's yeast is rapidly inactivated in the presence of N-acetylimidazole. According to kinetic data, acetylation of one amino acid residue of the protein per active site is sufficient for TK* inactivation. The holoenzyme is inhibited more slowly than is apotransketolase. The presence of a tyrosine residue in the enzyme's active site, essential for activity, is suggested.