Elimination of immune complex deposits from the kidneys of autoimmune NZB/N mice administered a xeno-spleen perfusate
Tsypin, A.B.; Nasonova, V.A.; Vedernikova, L.A.; Pleskovskaia, G.N.; Beletskaia, L.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 105(6): 703-705
1988
ISSN/ISBN: 0365-9615 PMID: 3390589 Document Number: 316316
54 autoimmune NZB/N mice, 6 and 10 months of age, were intravenously injected 0.2 ml solution prepared during perfusion of the isolated sheep spleen with a buffer solution. Perfusion solution was injected 8 times with 2-3-day intervals. The control group of 43 mice received intravenously 0.2 ml of a buffer solution in the similar manner. After the treatment the levels of anti-DNA antibodies and circulating immune complexes were significantly decreased in the sera of mice which received the perfusion solution, as compared with the levels of control groups. Immunofluorescent studies showed a marked decrease in the number of glomerular immune complexes deposits in mice treated with perfusion solution. Six- and ten-month old mice exhibited a similar effect. The perfusion solution may be capable of eliminating the immune complexes from the blood and kidneys of autoimmune mice.