Purification of murine liver-derived inhibitory protein
Wang, S.R.; Huang, M.H.; Chang, K.L.; Tsai, C.Y.; Lin, H.Y.; Yu, C.L.
Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 21(3): 179-187
1988
ISSN/ISBN: 0253-2662 PMID: 3149573 Document Number: 315043
Murine liver-derived inhibitory protein (LIP) capable of inhibiting human lymphocyte proliferation was separated from liver extract. The steps of purification included ammonium sulfate precipitation, DEAE-cellulose and hydroxylapatite ion exchange chromatographies, and gel filtration. The purified LIP appeared to be highly homogeneous, as verified by polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). There was arginase activity in the LIP Preparation.