Partial purification of porcine platelet-derived growth factor (PDGF)
Narczewska, B.; Czyrski, J.A.
Archivum Immunologiae et Therapiae Experimentalis 35(6): 831-837
1987
ISSN/ISBN: 0004-069X PMID: 3508040 Document Number: 305053
Fresh porcine blood platelets, subjected to ethanol acid extraction and purification on DEAE-Sephadex A-50 and CM-Sephadex C-50, were used to obtain partially purified, cationic PDGF (pI 8.5-10) of Mr 38,000 and biological activity 10(4) U/ml. Reduction of porcine PDGF with 2-mercaptoethanol revealed the presence of two polypeptides with Mr 18,000-22,000.
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