Distinct nuclear 7S RNAs hybridize to regulatory regions of two oncogenes
Kurz, J.; Lovely, J.; Cubitt, S.; Krause, M.O.
Biochemical and Biophysical Research Communications 152(2): 753-761
1988
ISSN/ISBN: 0006-291X PMID: 2835041 Document Number: 311742
The role of 7SK nuclear RNA remains obscure despite indications that it might be involved in the control of gene expression and associated with transformation. Here we identify distinct 7S nuclear transcripts by hybridization with DNA and RNA probes derived from regulatory regions of SV40 and c-myc genes. 7SK RNA hybridized to both early and late strands within the 21bp repeat region of SV4C and only to the sense strand within the first c-myc intron. Another nuclear 7S RNA, distinct from cytoplasmic 7SL, hybridized selectively to the SV40 late strand within the 72bp region. This RNA, designated 7SN, also hybridized to the c-myc first intron but with opposite polarity to 7SK. The identification of a novel 7S transcript and the observed selective hybridization of nuclear 7S RNAs with oncogene promoters raises interesting possibilities about their function.