Establishment of equine T-lymphocyte cultures dependent on recombinant human interleukin-2

Stott, M.L.; Osburn, B.I.

American Journal of Veterinary Research 49(4): 553-556

1988


ISSN/ISBN: 0002-9645
PMID: 3259849
Document Number: 310191
Long-term equine lymphocyte cultures were initiated and maintained in continuous culture with medium containing recombinant human interleukin-2. Cultures were successfully maintained with lectin activation signals and recombinant human interleukin-2 or with recombinant human interleukin-2 alone. All cell cultures that were characterized had a T-lymphocyte phenotype and had lectin-dependent or -independent cytotoxicity directed to various cell types. These findings demonstrate that long-term equine T-lymphocytes cultures can be initiated and maintained easily.

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