Dynamics of lipid accumulation on human aortic walls in atherosclerosis
Babaev, V.R.; Sirotkin, V.N.; Tararak, E.M.
Arkhiv Patologii 50(11): 35-40
1988
ISSN/ISBN: 0004-1955 PMID: 3069079 Document Number: 309850
The authors studied distribution of apoprotein B (apo B) and lipids containing esterified and nonesterified cholesterol (EC and NEC) in human aortic wall, using methods of indirect immunofluorescence with antibodies of apo B, and filipin and oil red 0 staining. All the examinees over 14 exhibited visually intact and atherosclerosis-affected vascular sites of accumulated apo-B-containing lipoproteins. EC, staining oil red 0, occurred only in the lipid strip and atherosclerotic plaque. Fillipin-positive NEC was found in atherosclerotic plaque. Filipin-positive NEC was found in atherosclerotic plaque only. NEC disseminated extracellularly in the form of separate spherical corpuscles and homogenously. Saline pretreatment of nonfixed cryostat sections reduced the intensity of filipin homogenous staining. Mechanisms of lipids formation in vascular tissue of humans are under discussion.