Determination of sulfobromophthalein excretion in rabbits
Quale, J.M.; Bergasa, N.V.; Mandel, L.J.
American Journal of Physiology 254(6 Pt 2): R1043-R1046
1988
ISSN/ISBN: 0002-9513 PMID: 3269212 Document Number: 309790
The excretion of sulfobromophthalein (BSP) is determined by hepatic uptake (storage capacity) and secretion (transport maximum). We calculated the BSP excretion data in six rabbits by measuring the rate of rise of BSP at various infusion rates. The relative storage capacity was determined to be 0.34 .+-. 0.13 mg .cntdot. mg-1 dl-1 .cntdot. kg-1. The transport maximum was calculated to be 0.91 .+-. 0.12 mg .cntdot. min-1 .cntdot. kg-1. Those results compare closely with previously reported resulting using more direct measurements that require anesthesia and surgical cannulation of the biliary tree. Repeated studies were easily performed. Our method provides a reliable and simple technique to study the effects of various conditions or substances on BSP excretion in conscious, unanesthetized rabbits.