Basophils exhibit rearrangement of the bcr gene in Philadelphia chromosome-positive chronic myeloid leukemia
Bosma, T.J.; Kennedy, M.A.; Bodger, M.P.; Hollings, P.E.; Fitzgerald, P.H.
Leukemia 2(3): 141-143
1988
ISSN/ISBN: 0887-6924 PMID: 3258049 Document Number: 306602
Basophils were isolated from the peripheral blood of two patients with Philadelphia positive-chronic myeloid leukemia using monoclonal antibody Bsp-1 and fluorescence activated cell sorting. DNA blot analyses demonstrated rearrangement of the breakpoint cluster region gene in the isolated basophils, which suggests their leukemic origin. Isolated T cells from these patients that were cultured for 14 days in the presence of interleukin-2 lacked rearrangement of the breakpoint cluster region gene and are therefore unlikely to be derived from the chronic myeloid leukemia clone.