Synthesis of human factor XIIIa in bacterial cells
Amann, E.; Abel, K.J.; Grundmann, U.; Okazaki, H.; Küpper, H.A.
Behring Institute Mitteilungen 82: 35-42
1988
ISSN/ISBN: 0301-0457 PMID: 2900632 Document Number: 306205
The coding sequence for human factor XIIIa (FXIIIa) was introduced into Escherichia coli expression vectors. Bacterial cells transformed with the recombinant plasmids synthesized fusion proteins of the expected molecular weights and the proteins were shown to be immunoreactive with anti-FXIII antibodies. Furthermore, with the help of oligodeoxynucleotide synthesis, we constructed a plasmid which directs the synthesis of the human FXIIIa protein in the unfused form. Sequence determination at the aminoterminus of this protein revealed the identical sequence compared to placental FXIIIa. The protein is expressed intracellularly in a denatured and biologically inactive form. It constitutes approximately 2% of total cellular protein and can easily be purified by standard methods.