Studies on the maintenance of antimicrobial agents in aqueous humor following topical administration
Tomii, T.
Nippon Ganka Gakkai Zasshi 92(5): 850-856
1988
ISSN/ISBN: 0029-0203 PMID: 3189066 Document Number: 305744
Aqueous humor levels of topically applied antimicrobial agents were determined following several dosage schedules in rabbit eyes. The agents consisted of 1% Disodium sulbenicillin (SBPC) solution, pH6.7MW 485.42, 0.3% Ofloxacin (OFLX) solution, pH 7.16MW 361.37, and 0.3% Sisomicin sulfate (SISO) solution, pH 7.0MW 692.71, were administered respectively to albino rabbit (2.0-2.5kg) eyes on the following schedules: a single instillation, one drop every 5min. for 10min., one drop every 5min. for 20 min., one drop hourly for 8h., one drop every 5 min. for 20,in. hourly for 8 hours. The drop size was approximately 40.mu.l. Aqueous humor samples were obtained for intervals up to 8 hours and examined by high performance liquid chromatography. Doses consisting of one drop every 5 min. for 10 or 20 min. produced higher levels in the aqueous humor than regimens using single instillations. Extended dose consisting of one drop hourly were observed to maintain low drug concentration levels in the aqueous humor up to 8 hours. However, one drop every 5 min. for 20 min. hourly for 8 hours produced remarkably high concentration in the aqueous humor. OFLX produced the highest penetration in the aqueous humor for a longer period than the other drugs.