Expression of the human insulin gene in transgenic mice
Jami, J.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 181(5): 475-490
1987
ISSN/ISBN: 0037-9026 PMID: 2966652 Document Number: 304922
Transgenic mice which specifically express the human insulin gene in the pancreatic beta cells have been obtained. Expression is demonstrated by the presence in the serum of human insulin (determined by a radioimmunoassay for the human C-peptide), the level of which increases upon glucose stimulus, and by the presence of human insulin mRNA in pancreas, but not in the other tissues tested. This result indicates that nucleotide sequences necessary for tissue-specific expression are within the transferred gene and/or in its vicinity. It also suggests that the regulatory molecules are quite similar if not identical in human and mouse.