The separation of unbound prednisolone in plasma by centrifugal ultrafiltration
Miller, P.F.; Wheeldon, J.; Brocklebank, J.T.
Journal of Pharmacy and Pharmacology 39(11): 939-941
1987
ISSN/ISBN: 0022-3573 PMID: 2892921 Document Number: 303425
To study variable plasma protein binding of prednisolone in children with nephrotic syndrome we have devises a simple rapid method for measuring unbound prednisolone. The plasma was initially ultrafiltered at 37.degree. C in a 35.degree. fixed angle heat at 1500 g for 30 min then then the filtrate was analysed by high pressure liquid chromatography. The effects of variable ultrafiltration conditions were studied. The method was used to compare the AUC unbound prednisolone in a nephrotic child (plasma albumin concentration 18 g L-1) with a control (plasma albumin concentration 43 g L-1); their respective AUC unbound prednisolone values were 4.02 mg h L-1 and 1.07 mg h L-1.