Rapid immunochemical determination of plasma fibronectin with a centrifugal analyzer
Hamel, G.; Toazara, D.J.; Mark, J.
Annales de Biologie Clinique 40(6): 661-665
1982
ISSN/ISBN: 0003-3898 PMID: 6819790 Document Number: 183959
We report a turbidimetric procedure, using commercially available reagents, for the determination of plasma fibronectin with an unmodified centrifugal analyser. Fixed-time kinetic readings at 340 nm were taken at 60 and 720 s. A wide range of concentration (up to 1000 mg/l) can be measured. Precision and accuracy were quite acceptable. Correlation with radial immunodiffusion was satisfactory. This automated procedure, simple and rapid, allows extensive studies of fibronectin in various diseases.