Top Abstract Purchase Related
Home
>
Node
>
Subnode

Effective method of isolating an acid-stable protease inhibitor from human urine

Ogloblina, O.G.; Belova, L.A.; Malakhov, V.N.

Voprosy Meditsinskoi Khimii 33(4): 119-124

1987

ISSN/ISBN: 0042-8809
PMID: 3660733
Document Number: 302447
A modification is described of the previously developed procedure for isolation of acid stable proteinase inhibitor (ASI) from urine of patients with nephritis. The modified procedure enabled to increase the yield of the inhibitor and to obtain its highly purified preparations. These preparations possessed a property to inhibit effectively the activity of human granulocyte elastase (ki = 10(4) M-1 min-1). The modified procedure is based on affinity chromatography on chymotrypsin-Sepharose 4B, substitution of dialysis by gel filtration on Sephadex G-25, use of alternative methods for concentration of protein fractions instead of membrane ultrafiltration and chromatography on polyamide for ASI depigmentation. The inhibitor yield amounted up to 75% of the initial activity in urine. The possibilities of the human urine ASI use in medical practice are discussed.

Document emailed within 1 workday
Secure & encrypted payments

Ogloblina, O.G.; Platonova, L.V.; Paskhina, T.S. 1979: Inhibition of human granulocyte proteinases by a heat and acid-stable proteinase inhibitor from rabbit serum Biokhimiia 44(4): 672-683
Della Porta, P.; Soeltl, R.; Krell, H.W.; Collins, K.; O'Donoghue, M.; Schmitt, M.; Krüger, A. 1999: Combined treatment with serine protease inhibitor aprotinin and matrix metalloproteinase inhibitor Batimastat (BB-94) does not prevent invasion of human esophageal and ovarian carcinoma cells in vivo Anticancer Research 19(5b): 3809-3816
Gaillard, M.C.; Reichberg, S.B.; Nogueira, C.M.; Kilroe-Smith, T.A. 1993: Differences in elastase-binding activity of alpha 1-protease inhibitor and alpha 2-macroglobulin for asthma patients and control subjects with various alpha 1-protease inhibitor phenotypes Clinical Chemistry 39(4): 675-679
Yasuda, T.; Nadano, D.; Tanaka, Y.; Sawazaki, K.; Kishi, K. 1991: Novel detection method for human protein C inhibitor (PCI) using immunoblotting with antibodies specific to synthetic peptides corresponding to the N-terminal and C-terminal amino acid sequences of human PCi Biochemistry International 25(3): 553-560
Chiba, M.; Hensleigh, M.; Nishime, J.A.; Balani, S.K.; Lin, J.H. 1996: Role of cytochrome P450 3A4 in human metabolism of MK-639, a potent human immunodeficiency virus protease inhibitor Drug Metabolism and Disposition: the Biological Fate of Chemicals 24(3): 307-314
Lillibridge, J.H.; Liang, B.H.; Kerr, B.M.; Webber, S.; Quart, B.; Shetty, B.V.; Lee, C.A. 1998: Characterization of the selectivity and mechanism of human cytochrome P450 inhibition by the human immunodeficiency virus-protease inhibitor nelfinavir mesylate Drug Metabolism and Disposition: the Biological Fate of Chemicals 26(7): 609-616
Zhong, L.; Batt, D.B.; Carmichael, G.G. 1994: An improved rapid method of isolating RNA from cultured cells by SDS-acid phenol/chloroform extraction Biotechniques 16(1): 56-57
Luber, A.D. 2005: Use of acid-reducing agents in protease inhibitor-based HAART and the potential for negative treatment outcomes Aids Reader 15(12): 692
Nartikova, V.F.; Paskhina, T.S. 1970: The kinetics of trypsin activity inhibition by a heat and acid-stable inhibitor in rabbit serum Biokhimiia 35(1): 187-195
Kubyshkin, A.V.; Ogloblina, O.G. 1992: Use of thermo- and acid-stable proteinase inhibitor to suppress proteolytic activation in pulmonary inflammation Biulleten' Eksperimental'noi Biologii i Meditsiny 113(6): 580-582
Watt, P.C.; Mullen, Y.; Benhamou, P.Y.; Hober, C.; Nomura, Y.; Watanabe, Y.; Passaro, E.P.; Zinner, M.J.; Brunicardi, F.C. 1994: Simplified semiautomated method for isolating islets from the human pancreas Transplantation Proceedings 26(2): 582-583
Skobelkin, O.K.; Bazhenova, G.E.; Kirpichev, A.G.; Ogloblina, O.G. 1991: Evaluation of the degree of trauma in biliary tract surgery using a laser method in relation to the status of the plasma protease inhibitor system Sovetskaia Meditsina 8: 52-54
Belozerov, A.P. 1989: A method of isolating C1q from human serum and its use in the solid-phase immunoenzyme determination of immune complexes Laboratornoe Delo 3: 24-26
Baranova, F.S.; Ogloblina, O.G.; Popova, L.K.; Platonova, L.V.; Berman, A.A. 1978: Property of a heat- and acid-stable inhibitor of serine proteinases from the blood serum to inhibit lymphcyte transformation stimulated by mitogens Biulleten' Eksperimental'noi Biologii i Meditsiny 85(5): 569-572
Yoshida, K.; Wago, K. 1981: Studies on the clinical significance of a simple fluorometric determination method of thiobarbituric acid reactive substance levels in human urine--urinary thiobarbituric acid reactive substance levels in hemiplegia and rheumatoid arthritis Rinsho Byori. Japanese Journal of Clinical Pathology 29(1): 87-89
Traber, D.L.; Adams, T.; Sziebert, L.; Williams, P.; Traber, L.D. 1984: Failure of a protease inhibitor to affect the cardiopulmonary response to endotoxin when combined with a cyclooxygenase inhibitor Circulatory Shock 13(4): 319-323
Petrova, I.V.; Vasil'eva, L.L. 1983: Method of isolating from human peripheral blood a pure population of neutrophils in order to study their rosette-forming properties Laboratornoe Delo 11: 26-28
Kuschak, D.; Mauss, S.; Schmutz, G.; Gantke, B. 2001: Simultaneous Determination of the New HIV Protease Inhibitor Lopinavir (ABT 378) and of Indinavir (1), Amprenavir, Saquinavir, Ritonavir (ABT 538) (2) and Nelfinavir (3) in Human Plasma by Gradient HPLC Clinical Laboratory 47(9-10): 471-477
Gaillard-Sanchez, I.; Bruneval, P.; Clauser, E.; Belair, M.F.; Da Silva, J.L.; Bariety, J.; Camilleri, J.P.; Corvol, P. 1990: Successful detection by in situ cDNA hybridization of three members of the serpin family: angiotensinogen, alpha 1 protease inhibitor, and antithrombin III in human hepatocytes Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 3(2): 216-222
Scuderi, P. 1989: Suppression of human leukocyte tumor necrosis factor secretion by the serine protease inhibitor p-toluenesulfonyl-L-arginine methyl ester (TAME) Journal of Immunology 143(1): 168-173