Immune response against P815X2 mastocytoma growing in syngeneic DBA/2 mice. IV. Lymph node immunoreactivity and cell-mediated cytotoxicity
Laatikainen, A.; Syrjänen, K.; Mäntyjärvi, R.; Tosi, P.
Neoplasma 34(4): 431-439
1987
ISSN/ISBN: 0028-2685 PMID: 3116439 Document Number: 299099
The in vitro cytotoxicity assay and the morphological in vivo analysis were used to follow the T cell reaction in tumor draining lymph nodes from DBA/2 mice bearing subcutaneously developing P815X2 mastocytoma. Mononuclear cells separated from local lymph nodes between days 8 and 14 following tumor inoculation were able to kill P815X2 cells in vitro. The maximal tumor-specific cytotoxicity was measured on day 10. Changes in lymph node morphology and in cellular contents of individual lymph node compartments were seen from day 12 onwards. The extensive expansion of the paracortex and the enlargement of the whole lymph node reached the peak on day 14. On days 12 and 14, a slight increase was also observed in the proportion of T lymphocytes. The results indicate that the tumor-specific cytotoxicity was detectable in tumor draining lymph nodes four days earlier than the morphological and cellular changes. The maximal cytotoxicity preceded the morphological peak reactions by two to four days.