Anomalous behaviour of cathepsin B: dependence of activity and stability on salt concentration
Khan, M.Y.; Ahmad, S.
Biochemistry International 15(1): 111-115
1987
ISSN/ISBN: 0158-5231 PMID: 3453681 Document Number: 298794
Effect on salt concentration on the activity and stability of cathepsin B from buffalo spleen has been investigated. Catheptic activity was maximal at a buffer concentration of 5.5 .times. 10-3 M. The enzyme was, however, highly unstable at a salt concentration lower than 1.5 .times. 10-2 M. The use of 0.02 M sodium phosphate buffer has been suggested for the assay of cathepsin B activity. For storage of the enzyme, however, a 0.1 M sodium phosphate or other buffer of equivalent ionic strength has been recommended.