Effect of chlorpromazine, dipyridamole and verapamil on platelet aggregation on the arachidonic acid metabolism
Zucchella, M.; Pacchiarini, L.; Grignani, G.
Bollettino della Societa Italiana di Biologia Sperimentale 63(12): 1137-1144
1987
ISSN/ISBN: 0037-8771 PMID: 3135826 Document Number: 298462
We studied the role of Ca++ and its mobilization through cell membranes on the following parameters: platelet aggregation induced by ADP or collagen and metabolism of arachidonic acid (AA) through cyclooxygenase or lipooxygenase pathways. Clorpromazine, dipyridamole and verapamil were used as inhibitors. These drugs were able to decrease significantly platelet aggregation and TxB2 production, while the production of LTC4 and LTB4 was unchanged. Our results suggest that Ca++ plays an important role in both primary and secondary platelet aggregation; furthermore, the metabolism of AA through ciclooxygenase pathway is involved in platelet aggregation; finally, the metabolism of AA through lipooxygenase pathway is present in platelets, but is not relevant in aggregation.