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Directed mutagenesis of chromosomal genes of Escherichia coli in vivo: construction of RecA- and HtpR- mutants

Kiselev, V.I.; Dubeĭkovskiĭ, A.N.; Pachkunov, D.M.; Astashkin, E.I.; Rechinskiĭ, V.O.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 43-46

1987

ISSN/ISBN: 0208-0613
PMID: 3037361
Document Number: 294884
The deletions in Escherichia coli chromosomal genes recA and htpR were constructed using the site-directed mutagenesis techniques. The obtained RecA- mutants are UV-sensitive and have a phenotype defective for the homologous DNA recombination. HtpR- mutant is temperature sensitive for growth and deficient in intracellular proteolysis. As a result a HtpR- mutant seems to be a preferable candidate for attempting to synthesize efficiently any alien protein in Escherichia coli cells.

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Hara, T.; Tanaka, T.; Kato, H.; Nishioka, T.; Oda, J. 1995: Site-directed mutagenesis of glutathione synthetase from Escherichia coli B: mapping of the gamma-L-glutamyl-L-cysteine-binding site Protein Engineering 8(7): 711-716
Kiselev, V.I.; Pachkunov, D.M.; Astashkin, E.I.; Kiselev, O.I. 1987: Regulation of expression of the htpR gene in Escherichia coli Molekuliarnaia Biologiia 21(4): 1050-1055
Kiselev, V.I.; Tarasova, I.M. 1988: Regulation of proteolysis in Escherichia coli cells by antisense RNA of htpR gene Biotechnology and Applied Biochemistry 10(1): 59-62
Kumar, S.; Prakash, N.; Agarwal, K.N. 1979: Cyclic AMP control of the envelope growth in Escherichia coli: envelope morphology of the mutants in cya and crp genes Indian Journal of Experimental Biology 17(4): 325-327
Zavil'gel'skiĭ, G.B.; Eroshnikov, G.E.; Manukhov, I.V.; Rasulova, F.S.; Ginodman, L.M.; Mel'nikov, E.E.; Starkova, N.N.; Rotanova, T.V. 1999: In vivo analysis of the proteolytic activity and effects of "sequestering" and negative domination of Escherichia coli lon-mutants using the lux regulon of Vibrio fischeri Molekuliarnaia Biologiia 33(5): 797-802
McCracken, A.A.; Kruse, K.B.; Brown, J.L. 1988: An enrichment selection for mutants resulting from oligonucleotide-directed mutagenesis of double-stranded DNA Biotechniques 6(4): 332-339
Verbenko, V.N.; Kuznetsova, L.V.; Krup'ian, E.P.; Shalguev, V.I. 2009: Expression of gene recA of Deinococcus radiodurans in Escherichia coli cells Genetika 45(10): 1353-1360
Suchkov, I.I.; Mishan'kin, B.N. 1989: Cloning and study of the function of recA-like gene of Yersinia pestis in Escherichia coli cells Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 34-39
Matejczyk, M.; Swislocka, R.; Kalinowska, M.; Swidersk, G.; Lewandowsk, W.; Jablonska-Trypuo, A. 2017: Monitoring of Synergistic Enhancement of Caffeic Acid on Escherichia Coli K-12 Reca::gfp Strain Treated with Dacarbazine Acta Poloniae Pharmaceutica 74(3): 809-816
Ivasiuk, L.A. 1975: Influence of the recA- mutation on spontaneous filamentation and the titer of the lambda phage in ylonB- mutants of E. coli K12 (lambda) Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 95-97
Macphee, D.G.; Liaskou, D. 1989: Mutagenesis by the anti-tumour drug nitracrine in Escherichia coli Mutation Research 227(4): 251-255
Shakulov, R.S.; Kliachko, E.V.; Ponomarenko, O.I. 1985: The role of pp Gpp in the coordination of transcription of the ribosomal protein genes rplKAJL and RNA-polymerase rpoBC genes in Escherichia coli cells Molekuliarnaia Biologiia 19(6): 1603-1609
Mashko, S.V.; Lapidus, A.L.; Trukhan, M.E.; Lebedeva, M.I.; Podkovyrov, S.M. 1987: Creation of artificial hybrid operons with partially overlapping genes to achieve an expression of heterologous genes in Escherichia coli cells Molekuliarnaia Biologiia 21(5): 1297-1309
Kocharian, S.M.; Livshits, V.A.; Sukhodolets, V.V. 1975: Genetic study of Escherichia coli K-12 mutants resistant to 2,6-diaminopurine Genetika 11(11): 79-89
Wood, P.A.; Farmer, S.C.; Tolwani, R.J.; Warren, J.R.; Steinkampf, M.P.; Johnson, L.W.; Mountz, J.D.; Kelly, D.P. 1992: Molecular studies of mouse medium and long-chain acyl-CoA dehydrogenase genes for site-directed mutagenesis of embryonic stem cells Progress in Clinical and Biological Research 375: 151-160
Orozco de Silva, A.; Toro, S. 1990: Isolation and characterization of mutants affected in the expression of the nar operon Escherichia coli Acta Cientifica Venezolana 41(1): 21-25
Soĭfer, V.N.; Rkhkian, L.A.; Titov, I.U. 1976: Mutagenesis of synchronous populations of Escherichia coli with normal and defective reparative enzymes under the influence of hydroxylamine Mikrobiologiia 45(1): 151-156
Upmal, M.R.; Kobets, N.S.; Il'ina, T.S.; Piruzian, E.S. 1984: Genetic analysis of the Mud mutants of Escherichia coli K-12 with an impaired ability to maintain bacteriophage Mu development Genetika 20(6): 924-932
Oethinger, M.; Podglajen, I.; Kern, W.V.; Levy, S.B. 1998: Overexpression of the marA or soxS regulatory gene in clinical topoisomerase mutants of Escherichia coli Antimicrobial Agents and Chemotherapy 42(8): 2089-2094
Ueguchi, C.; Ito, K. 1991: The sec genes of Escherichia coli Seikagaku. Journal of Japanese Biochemical Society 63(4): 293-298