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Effect of pH on antigen binding by clonotypic antibodies with different isoelectric points

Endo, Y.; Miyai, K.; Hata, N.; Iijima, Y.

Biotechnology and Applied Biochemistry 9(1): 74-81

1987

ISSN/ISBN: 0885-4513
PMID: 3566921
Document Number: 293892
Polyclonal rabbit antibodies to thyroxine, human myoglobin, human growth hormone, human thyrotropin, human .alpha.-fetoprotein, and human thyroglobulin were fractionated into clonotypic antibodies with different isoelectric points by agarose isoelectric focusing or chromatofocusing. The effect of pH on the binding of these antigens by their respective clonotypic antibodies was assessed by radioimmunoassay. The profiles of the pH effect differed both for different antigens and for different pI's of the antibodies used. The pH optima in the radioimmunoassays for protein antigens were found to be expressed as a function of pI and molecular weight of both antigen and antibody molecules.

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Cheng, W.C. 1971: The reaction of immunoglobulin-coated bentonite and radioiodinated antigen. II. Quantitative antigen-binding studies of antibodies and selectively isolated antibodies to 2,4-dinitrobenzene, 2,4-dinitro-5-aminobenzene and 2,4,6-trinitrobenzene Journal of Immunology 106(1): 150-159
Agius, M.A.; Geannopoulos, C.J.; Fairclough, R.H.; Richman, D.P. 1988: Monoclonal anti-idiotopic antibodies against myasthenia-inducing anti-acetylcholine receptor monoclonal antibodies. Preponderance of nonparatope-directed antibodies affecting antigen binding Journal of Immunology 140(1): 62-68
Malamud, D.; Drysdale, J.W. 1978: Isoelectric points of proteins: a table Analytical Biochemistry 86(2): 620-647
Legendre, J.M.; Bergot, A.; Turzo, A.; Morin, P.P. 1993: Changes in isoelectric points of the monoclonal antibody OC 125 during indium 111 radiolabeling Pathologie-Biologie 41(2): 169-171
Benfari, M.J.; Cooperband, S.R.; Moolten, F.L. 1977: Characterization of antigen-binding receptors in vitro. I. An equilibrium method for measuring antigen binding in murine cells Journal of Immunology 119(4): 1427-1431
Gillies, S.D.; Wesolowski, J.S. 1990: Antigen binding and biological activities of engineered mutant chimeric antibodies with human tumor specificities Human Antibodies and Hybridomas 1(1): 47-54
Mel'nikova, I.I.; Lunev, V.E.; Preĭgerzon, V.A.; Luneva, N.M.; Koshkin, S.A.; Rodionov, M.A.; Martsev, S.P. 1993: Monoclonal antibodies to human spleen ferritin. II. Localization of epitopes and quantitative parameters of antigen binding Biokhimiia 58(5): 759-771
Freisenhausen, H.D.; Finke, M.; Bülow, B.; Baals, H.; Frahm, H. 1972: Simultaneous incidence of Australia (SH) antigen and complement binding antibodies against Cytomegalovirus in blood donors in Hamburg Die Medizinische Welt 23(49): 1851-1853
Berezin, V.E.; Taran, O.I.; Zubov, S.V.; Zaĭdes, V.M.; Anan'ev, V.A. 1986: Binding of the surface antigen of the hepatitis B virus with antibodies studied with different denaturation exposures and with different analytical methods Voprosy Virusologii 31(4): 452-456
Kiriukhin, I.F.; Bagdasar'ian, S.N. 1971: Study by the peptide map method of G-globulin fractions of bovine blood serum and lymph node tissue proteins with corresponding isoelectric points Biulleten' Eksperimental'noi Biologii i Meditsiny 72(12): 34-37
Guzov, V.M.; Usanov, S.A. 1988: Optimization of methods for immobilizing antibodies against the carcino-embryonic antigen on insoluble matrices. Equilibrium parameters of the interaction of the immobilized antibodies with the antigen Biokhimiia 53(11): 1772-1782
Horejsí, V.; Angelisová, P.; Bazil, V.; Kristofová, H.; Stoyanov, S.; Stefanová, I.; Hausner, P.; Vosecký, M.; Hilgert, I. 1988: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74) Folia Biologica 34(1): 23-34
Morrow, C.D.; Dorey, F.; Stevens, R.H. 1983: Isoelectric focusing of human anti-diphtheria toxoid antibodies: identical spectrotypes of anti-fragment a antibodies with the same IgG subclass and light chain constant regions are expressed in multiple individuals Journal of Immunology 130(2): 818-823
van Eijk, H.G.; van Noort, W.L.; Kroos, M.J.; van der Heul, C. 1978: Analysis of the iron-binding sites of transferrin by isoelectric focussing Journal of Clinical Chemistry and Clinical Biochemistry 16(10): 557-560
Ditzel, H.; Erb, K.; Leslie, G.; Jensenius, J.C. 1993: Preparation of antigen-binding monomeric and half-monomeric fragments from human monoclonal IgM antibodies against colorectal cancer-associated antigens Human Antibodies and Hybridomas 4(2): 86-93
Matsuda, T.; Kabat, E.A. 1989: Variable region cDNA sequences and antigen binding specificity of mouse monoclonal antibodies to isomaltosyl oligosaccharides coupled to proteins. T-dependent analogues of alpha(1----6)dextran Journal of Immunology 142(3): 863-870
Tarakanova, I.N.; Dmitriev, D.A.; Massino, I.S.; Smirnova, M.B.; Segal, O.L.; Fartushnaia, O.V.; Iakovleva, D.A.; Koliaskina, G.I.; Lavrov, V.F.; Dmitriev, A.D. 2012: Effect of conditions of monoclonal antibody adsorption on antigen-binding activity Prikladnaia Biokhimiia i Mikrobiologiia 48(5): 557-563
Toyoda, T.; Osumi, M.; Aoyagi, T.; Kawashiro, T. 1996: Serodiagnosis of tuberculosis by detection of antituberculous glycolipid antigen (TBGL antigen) antibodies in serum using enzyme-linked immunosorbent assay: clinical evaluation of anti-TBGL antibodies assay kit Kekkaku: 71(12): 655-661
Thomas, J.W.; Virta, V.J.; Nell, L.J. 1985: Heterogeneity and specificity of human anti-insulin antibodies determined by isoelectric focusing Journal of Immunology 134(2): 1048-1052
Davie, J.M.; Paul, W.E.; Green, I. 1972: Genetic control of the immune response of inbred guinea pigs to 2,4-dinitrophenyl guinea pig albumin. Frequency of antigen-binding lymphocytes and avidity of antibodies secreted by plaque-forming cells Journal of Immunology 109(2): 193-200