Effect of the restriction of nitrogen intake on muscle protein synthesis in experimental uremic rats
Fujii, M.; Ando, A.; Mikami, H.; Okada, A.; Imai, E.; Kokuba, Y.; Yamauchi, A.; Orita, Y.; Kamada, T.; Abe, H.
Kidney International. Supplement 22: S153-S158
1987
ISSN/ISBN: 0098-6577 PMID: 2448517 Document Number: 291350
Uraemic Sprague-Dawley rats weighing 140 to 150 g were given, as total parenteral nutrition, 2 isoenergetic diets with nitrogen 848 or 1696 mg/kg body weight and with a constant non-protein energy input of 265 kcal/kg body weight. To assess the protein-synthesizing activity (PSA) of muscle, RNA content, polysome profiles and cell-free PSA of the polysomes were examined. Moderately uraemic rats conserved their PSA in muscle. The N restriction, as in controls, decreased their muscle PSA. In severely uraemic rats the PSA of muscle was less than that of the control and moderately uraemic groups. The N-restricted diet which decreased PSA in muscle of controls and moderately uraemic rats did not exacerbate the PSA in the muscle of severely uraemic rats.