Single channel recordings from basolateral membranes of frog urinary bladders
Parisi, M.; Uchitel, O.
Acta Physiologica et Pharmacologica Latinoamericana Organo de la Asociacion Latinoamericana de Ciencias Fisiologicas y de la Asociacion Latinoamericana de Farmacologia 37(4): 485-490
1987
ISSN/ISBN: 0326-6656 PMID: 2483999 Document Number: 291004
A methodology is described to obtain, in frog urinary bladder, isolated epithelial layers suitable for patch clamp studies. This was achieved combining negative pressure, enzymatic digestion, and microdissection. Single channels recordings were obtained from the basolateral membranes while the tissue maintained its general structure and polarity.