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Optimization of the immunofluorescence test for antibodies in the serum of patients with inner ear diseases

Wei, N.R.; Giebel, W.

Laryngologie Rhinologie Otologie 66(1): 6-10

1987

ISSN/ISBN: 0340-1588
PMID: 3550338
Document Number: 290411
Antibodies in the serum of patients with inner ear diseases can be demonstrated immunohistochemically with human kidney tissue. In this study a patient's serum with the typical positive reaction against proximal tubuli (antibodies against microsomes) and a serum with antibodies against mitochondria was tested with different dilutions of the fluorescein marked antiserum (FITC conjugate). For both sera a dilution of 1:160 showed positive reaction. The fluorescence of the control incubation was negligible. Employing the given technical details the immunohistochemical assay of antibodies with human kidney tissue has become a simple and reliable test for the most frequently found antibodies in patients with inner ear diseases.

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