Submerged-phase gel electrophoresis in the analysis of plasmids for epidemiologic purposes
Caldini, G.; Cenci, G.; Morozzi, G.
Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 23(3): 274-286
1987
ISSN/ISBN: 0033-4979 PMID: 3332378 Document Number: 286269
The validity of the submarine agarose gel electrophoresis as routine method for plasmidic epidemiology was considered. Using standard plasmids, the efficiency of a performed protocol conditions (5 V/cm for 3 hours, 0.8% agarose) in screening plasmids of different size was studied. The results provide sharp profile resolution and an accurate estimate of molecular weights in the range from 1.2 to 112 megadaltons.