Delayed alteration of membrane fluidity in intact cultured B-16 melanoma cells affected by ultraviolet irradiation
Sakanashi, T.; Sugiyama, M.; Suematsu, T.; Hidaka, T.; Ogura, R.
Biochemistry International 12(2): 341-346
1986
ISSN/ISBN: 0158-5231 PMID: 3008753 Document Number: 283675
Lipid peroxidation in the plasma membrane has been reported to decrease membrane fluidity. We examined membrane fluidity in relation to lipid peroxidation processes after UV-B exposure of cultured B-16 melanoma cells. UV exposure promptly increased TBA-positive material(s), but alteration of membrane fluidity was delayed. Plasma membrane fluidity increased significantly 6 hours after exposure when the TBA-value(s) had become under the control level. To examine the direct effect of lipid peroxides on the fluidity, tert-butyl hydroperoxide was added to B-16 melanoma cells. Similar results were obtained with respect to membrane fluidity. These results suggest that lipid peroxidation at UV doses maintaining cell viability does not directly induce a significant alteration of membrane fluidity, but may influence the fluidity either during metabolizing processes of UV-induced lipid peroxides or during repair processes following oxidative cell membrane damage.