Membrane fluidity in Ehrlich ascites tumor cells treated with adriamycin
Sugiyama, M.; Sakanashi, T.; Okamoto, K.; Chinami, M.; Hidaka, T.; Ogura, R.
Biotechnology and Applied Biochemistry 8(2-3): 217-221
1986
ISSN/ISBN: 0885-4513 PMID: 3741633 Document Number: 267985
The incubation of Ehrlich ascites tumor cells with adriamycin resulted in an increase in lipid peroxide content and a decrease in membrane fluidity as measured by electron spin resonance using the paramagnetic probe 5-doxylstearic acid. Coincidently, the incorporation of [3H]thymidine into tumor cells was progressively inhibited as the concentration of adriamycin was increased. The results indicate that adriamycin induces changes in the plasma membrane of Ehrlich ascites tumor cells after exposure to a low, but cytotoxic, level of this agent.