Effect of dietary columbinic acid on the fatty acid composition and physical membrane properties of different tissues of EFA-deficient rats
Mandon, E.C.; de Gómez Dumm, I.N.; Brenner, R.R.
Archivos Latinoamericanos de Nutricion 36(3): 401-414
1986
ISSN/ISBN: 0004-0622 PMID: 3632215 Document Number: 279646
For 1 month 24 male weanling Wistar rats were given a balanced diet or a fat-free diet. Then those on the fat-free diet were kept on that diet or were given it supplemented with 1.5% columbinic acid (5-trans,9-cis,12-cis-octadecatrienoic acid) for 24 or 48 h. The fat-free diet produced changes in fatty acid composition of tissues characteristic of essential fatty acid deficiency. Columbinic acid was incorporated into the different tissues and was partly elongated to 7-trans,11-cis,14-cis-eicosatrienoic acid but it was not desaturated. It modified the fatty acid spectrum of the lipids in different tissues returning it to a similar composition to that in normal rats, except for a decrease in linoleic acid. The absence of lipids in the diet produced an increase in the 1-6-diphenyl-1,3,5-hexatriene steady-state fluorescence anisotropy in liver microsomes, that was corrected by columbinic acid given for 24 h.