Comparison of two methods for determination of magnesium concentration of mononuclear blood cells
Elin, R.J.; Hook, G.; Hosseini, J.M.
Magnesium 5(5-6): 301-305
1986
ISSN/ISBN: 0252-1156 PMID: 3807423 Document Number: 274523
We describe and compare two methods for the determination of magnesium concentration in lymphocytes from cell cultures. The magnesium concentration of lymphocytes was 36 .+-. 4 mmol/kg dry weight by atomic absorption spectroscopy analysis and 39 .+-. 19 mmol/kg dry weight with electron probe X-ray microanalysis. We found no significant difference between the results for the two methods. The coefficient of variation was 30% for magnesium concentration among individual cells with electron probe X-ray microanalysis.
Document emailed within 1 workday
Related Documents
Huang, C.M.; Fleisher, T.A.; Elin, R.J. 1988: Magnesium content and concentration of mononuclear and T lymphocyte blood cells Magnesium 7(4): 219-224Gryboś, M.; Krzemieniewska, J.; Stacherzak-Pawlik, J.; Wilczyński, A.; Woźniak, M.ła.; Majsnerowicz, M.; Gryboś-Jagielska, A. 2005: Total and ionized magnesium concentration in the blood plasma and erythrocytic magnesium concentration of women in the third trimester of pregnancy with imminent preterm labor Ginekologia Polska 76(8): 625-631
Hook, G.R.; Hosseini, J.M.; Elin, R.J. 1988: Density gradients for isolation of mononuclear blood cells for magnesium analysis by electron probe X-ray microanalysis Magnesium 7(1): 1-5
Wood, R.; Dong, H.; Katzenstein, D.A.; Merigan, T.C. 1993: Quantification and comparison of HIV-1 proviral load in peripheral blood mononuclear cells and isolated CD4+ T cells Journal of Acquired Immune Deficiency Syndromes 6(3): 237-240
Luo, X.; Rong, Y.; Wang, L.; He, J.; Zhao, M.; Lu, W.; Zhang, R.; Sun, W.; Chen, W. 2015: Comparison of two methods for fiber count concentration determination Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 33(11): 822-825
De Maddalena, C.; Zehender, G.; Bianchi Bosisio, A.; Monti, G.; Monteverde, A.; Invernizzi, F.; Galli, M. 1995: HCV-RNA detection using different PCR methods in sera, cryoglobulins and peripheral blood mononuclear cells of patients with mixed cryoglobulinemia Clinical and Experimental Rheumatology 13(Suppl 13): S119-S122
Longatto Filho, A.; Maeda, M.Y.; Santos, D.R.; de Andréa Filho, A.; Cavaliere, M.J.; Shih, L.W.; Oyafuso, M.S. 1991: Comparison of the methods of cytobrush and Ayre spatula in the concentration of endocervical cells Revista Paulista de Medicina 109(3): 93-96
Robson-Ansley, P.J.; Saini, A.; Toms, C.; Ansley, L.; Walshe, I.H.; Nimmo, M.A.; Curtin, J.A. 2014: Dynamic changes in dna methylation status in peripheral blood Mononuclear cells following an acute bout of exercise: Potential impact of exercise-induced elevations in interleukin-6 concentration Journal of Biological Regulators and Homeostatic Agents 28(3): 407-417
Meister, W.; Klemm, J.; Kriener, J.; Mayer, L.; Rudolph, W. 1971: Comparison of various methods used in the determination of myocardial blood flow in man Verhandlungen der Deutschen Gesellschaft für Innere Medizin 77: 441-445
Ratto-Kim, S.; Sitz, K.V.; Scherer, A.M.; Kim, J.H.; Anderson, D.W.; Nau, M.E.; Mann, D.L.; Akolkar, P.N.; Gulwani-Akolkar, B.; Silver, J.; Birx, D.L. 1997: Comparison of HIV-1 envelope-specific CD4+ T cell lines simultaneously established from peripheral blood mononuclear cells and lymph node biopsy in HIV-1-infected individuals Journal of Immunology 159(10): 5162-5167
Shabunin, I.V.; Shimanovskiĭ, N.L.; Panov, V.O.; Kalinina, A.A.; Sviridov, N.K. 1999: The effect of x-ray contrast media on the concentration of intracellular calcium in mononuclear cells Eksperimental'naia i Klinicheskaia Farmakologiia 62(6): 62-65
De la Concha-Bermejillo, A.; Schore, C.E.; Dangler, C.A.; de Mattos, C.C.; de Mattos, C.A.; Osburn, B.I. 1992: Comparison of slot blot nucleic acid hybridization, immunofluorescence, and virus isolation techniques to detect bluetongue virus in blood mononuclear cells from cattle with experimentally induced infection American Journal of Veterinary Research 53(12): 2245-2250
Buratti, M.; Pellegrino, O.; Soardo, A.; Colombi, A. 1990: Determination of blood bile acids using high pressure liquid chromatography: comparison of chromatographic, enzymatic and immunoenzymatic methods La Medicina del Lavoro 81(1): 65-74
Morita, K.; Kimura, H. 1998: Simple determination methods for blood grouping from biological stains. 2. Immunochromatographic determination of ABO blood groups from saliva specimens Nihon Hoigaku Zasshi 52(3): 191-195
Holm, V. 1983: Further studies of the concentration of magnesium ion (Mg++) in blood from patients with autoimmune diseases Danish Medical Bulletin 30(3): 180-184
Wang, K.; Chen, L.Y.; Wang, B.; Han, L.Y.; Hou, Y.D. 2006: Phenotype of peripheral blood mononuclear cells derived dendritic cells from patients with chronic hepatitis B Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 20(3): 250-253
Mahfouz, M.M.; Smith, T.L.; Kummerow, F.A. 1992: Decreased uptake of low density lipoprotein by LLC-PK cells cultured at low magnesium concentration Magnesium Research 5(4): 249-254
Tomonari, K.; Aizawa, M. 1979: Induction of autoreactive cells by the preculture of human peripheral blood mononuclear cells with the autologous fresh plasma Journal of Immunology 122(6): 2478-2483
Radzivil, G.G.; Mikheeva, E.V.; Kutasova, I.V. 1975: Study of total magnesium concentration in the blood of patients after surgery with extracorporeal circulation Kardiologiia 15(4): 118-123
Satake, K.; Lee, J.-D.; Shimizu, H.; Uzui, H.; Mitsuke, Y.; Yue, H.; Ueda, T. 2004: Effects of magnesium on prostacyclin synthesis and intracellular free calcium concentration in vascular cells Magnesium Research 17(1): 20-27