Activation of calcium transport in synaptosomes by phosphatidylinositol-specific phospholipase C
Brusovanik, V.I.; Selishcheva, A.A.; Prilipko, L.L.; Kozlov, I.P.; Kulene, V.V.
Biokhimiia 51(8): 1329-1333
1986
ISSN/ISBN: 0320-9725 PMID: 3768437 Document Number: 272436
The isotope labeling method was used to study the influence of phospholipases C of different origin and specificity on Ca2+ accumulation in rat brain synaptosomes. It was found that phospholipases C specific to phosphatidylinositides (PI) stimulate Ca2+ transport into synaptosomes, while non-specific phospholipase C, which hydrolyzes different membrane lipid fractions, decreases the Ca2+ content in synaptosomes. It is supposed that the stimulating effect of PI-specific phospholipases C is determined by the activation of PI metabolism, which results in an increase in the content of some PI metabolism products serving as Ca2+ ionophores in synaptosomal membranes. The inhibition of Ca2+ uptake by synaptosomes treated with non-specific phospholipase C is thought to result from partial disruption of synaptosomal membranes.