Evaluation in vitro of the antimicrobial activity of two topical preparations used in the management of ear infections in the dog
Lloyd, D.H.; Lamport, A.I.
Veterinary Therapeutics Research in Applied Veterinary Medicine 1(1): 43-47
2000
ISSN/ISBN: 1528-3593 PMID: 19757564 Document Number: 270
Products designed for ear cleansing (i.e., Epi-Otic and Epi-Otic New Formula [NF]) were evaluated in vitro for their ability to kill clinical isolates of Staphylococcus intermedius, Pseudomonas aeruginosa, and the yeast Malassezia pachydermatis. Dilutions of the products at 1/5 and ½5 in distilled water were inoculated with suspensions of the organisms and held at room temperature. The mixtures were sampled at intervals between 1 and 16 minutes, and numbers of viable colonyforming units of the microorganisms were determined by culture on blood agar or modified Dixon's agar (for the yeast) using the spreadplate method. At a dilution of 1/5, both products were able to eliminate S. intermedius within 1 minute. P aeruginosa was eliminated within 2 minutes by both products. M pachydermatis was eliminated by Epi-Otic NF and Epi-Otic within 4 and 8 minutes, respectively. Lower activity was demonstrated following dilution at ½5. The findings support previously published data for Epi-Otic indicating antimicrobial activity both in vitro and in vivo. The findings suggest that Epi-Otic may be useful in the control of ear infections.
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