CS antigen localization in malaria vectors: hypothetical refractoriness to transmission observed in the field
Lombardi, S.; Esposito, F.; Zavala, F.; Lamizana, L.; Rossi, P.; Sabatinelli, G.; Nussenzweig, R.S.; Coluzzi, M.
Parassitologia 28(2-3): 113-116
1986
ISSN/ISBN: 0048-2951 PMID: 3332304 Document Number: 269956
Indoor resting Anopheles gambiae s.l. were collected in 2 villages near Ouagadougou, Burkina Faso, and processed to investigate the presence and distribution of Plasmodium falciparum sporozoites. Salivary glands were dissected, examined by phase contrast microscopy and further processed by IRMA in order to reveal the presence of the circumsporozoite (CS) antigen of P. falciparum. The corresponding thoraces were homogenized and processed by IRMA. In the village characterized by the higher inoculation rate, >40% of the infected mosquitoes were not infective; CS antigen was detected in the thorax in the absence of sporozoites and CS antigen in the corresponding salivary glands.