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Analysis of ribonucleoproteins of influenza virus containing genomic and complementary RNA

Boshchenko, I.A.; Farashian, V.R.; Zaĭdes, V.M.; Zhdanov, V.M.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1985(9): 32-36

1985

ISSN/ISBN: 0208-0613
PMID: 3842758
Document Number: 264306
The density and sedimentation characteristics of ribonucleoproteins (RNP) containing genomic RNA from influenza virus and RNA complementary have been studied. Radioactive RNA from infected cells has been used for analysis. RNA classes of interest were isolated by reannealing with abundant nonradioactive genomic and complementary RNA and separation of resulting duplexes in electrophoresis. The RNP containing antigenomic virus-specific RNA are practically identical to "genomic" RNP for their sedimentation and density characteristics. The "plus" RNP is characterized by the stoichiometric mode of RNA protein interaction.

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