Abnormal B cell function in haemophiliacs and their relationship with factor concentrates administration
Brieva, J.A.; Sequi, J.; Zabay, J.M.; Pardo, A.; Campos, A.; Luz de la Sen, M.; Bootello, A.
Clinical and Experimental Immunology 59(2): 491-498
1985
ISSN/ISBN: 0009-9104 PMID: 3919981 Document Number: 262546
B cell function was evaluated in hemophiliacs. Spontaneous and pokeweed mitogen (PWM)-induced Ig production was determined by ELISA [enzyme-linked immunosorbent assay] in the supernatants of cultured peripheral blood lymphocytes (PBL) from 14 hemophiliacs and 17 normal donors. Spontaneous IgM, IgA and IgG production was 3 times higher in patients than normal controls, while PWM-induced IgM, IgA and IgG production was markedly reduced in patients compared to normal donors (P < 0.025). Allogeneic co-cultures of hemophiliacs and normal B plus T cell fractions revealed that these results are due to a defect of the patients' T cell depleted fraction. These abnormalities were not found in 3 patients who had received no clotting factor concentrates for at least 1 yr prior to the study. The annual amount of clotting factor concentrates received by treated patients correlates well with the enhancement of spontaneous Ig production (r = +0.688, P < 0.02), the decrease of PWM-induced Ig secretion (r = -0.655, P < 0.02), and the elevation of serum IgG levels (r = +0.610, P < 0.05). These findings suggest that the administration of clotting factor concentrates play an important role in the altered B cell function in hemophiliacs.