Relationship between structure and function of a chemotactic factor for human leukocytes
Carletti, R.; Spisani, S.; Traniello, S.
Bollettino della Societa Italiana di Biologia Sperimentale 57(22): 2257-2263
1981
ISSN/ISBN: 0037-8771 PMID: 7326113 Document Number: 181394
Casein, a phosphoprotein forming aggregates in solution, exerts chemotactic activity for human neutrophils. The protein was filtered on Sephadex G 100 to obtain fractions of homogeneous mol. weight and the column fractions were tested for chemotactic activity. The chemotactic activity was found only in the lighter peak, which was then digested by trypsin. The digested material was purified by filtration through AcA-54 and the 6.000 M.W. peptide containing the most phosphate groups and tyrosines resulted chemotactic for PMN. Most likely the phosphate groups are important in chemotactic recognition.