Identification of the number of mutant copies of the genetic transfer factor AP42

Gubar', E.V.; Pekhov, A.P.

Biulleten' Eksperimental'noi Biologii i Meditsiny 100(8): 223-226

1985


ISSN/ISBN: 0365-9615
PMID: 2992634
Document Number: 258292
The copy number mutant of genetic transfer factor pAP42 (incFIX) was induced by the treatment of E. coli AP115 cells carrying the plasmid by N-methyl-N-nitro-N-nitrosoguanidine. The copy number mutant pAP42::Tn1cop1 is characterized by the increased copy number. The cells carrying the copy number mutant have a higher ampicillin resistance and higher beta-lactamase activity. Mutant plasmid pAP42: :Tn1cop1 is incompatible with plasmid pAP42 and compatible with plasmids of the other inc-groups of F-like plasmids.

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