The effect of retinol deficiency on the metabolism of all-trans-retinyl acetate in rat testes
Chaudhary, L.R.; Nelson, E.C.
International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 55(4): 383-390
1985
ISSN/ISBN: 0300-9831 PMID: 4086206 Document Number: 258049
Following intratesticular injection, the metabolism of all-trans-[11-3H] retinyl acetate was studied in rats fed a vitamin A-deficient diet supplemented either with retinyl palmitate (-A + RP) or retinoic acid (-A + RA). Analysis of testicular metabolites by HPLC at 6 h and 24 h demonstrated the hydrolysis of retinyl acetate to retinol, esterification of retinol to retinyl palmitate and the formation of trace amounts of retinoic acid and other metabolites in both groups of rats. Eight and nine metabolite peaks were present in the -A + RP and -A + RA groups, respectively. The HPLC profile was similar in both groups of rats but the amounts of metabolites differed.