A comparison of methods of monoclonal immunoglobulin quantitation
Tichý, M.
Neoplasma 32(1): 31-36
1985
ISSN/ISBN: 0028-2685 PMID: 3920541 Document Number: 254048
Quantitation of monoclonal Ig was carried out using the following methods: densitometric scanning of electrophoretograms on cellulose acetate gel, single radial immunodiffusion, nephelometry and, in monoclonal IgM, also sedimentation analysis. To compare the methods, 20 sera with monoclonal IgG, 16 sera with monoclonal IgA and 19 sera with monoclonal IgM were used. The methods correlated well in monoclonal IgM and IgG, while in IgA correlation was found only between densitometry and radial immunodiffusion. Closest correlation was observed between densitometry and sedimentation analysis in quantifying monoclonal IgM (r = 0.954). Despite good correlation, the individual values of monoclonal Ig established by different methods showed marked differences. In comparison with the immunochemical methods, the quantitation of monoclonal Ig using densitometric scanning of electrophoretograms is accessible, quick and a relatively more precise method. [Quantitation of monoclonal Ig is of importance mainly for differential diagnosis of monoclonal gammopathies.].