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Cultivation of Neisseria meningitidis serogroups A, B and C in a synthetic nutrient broth

Karabak, V.I.; Basnak'ian, I.A.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 11: 6-10

1985

ISSN/ISBN: 0372-9311
PMID: 3937394
Document Number: 253601
The processes of the cultivation of N. meningitidis, serogroups A, B and C, in a liquid synthetic culture medium have been studied. Strictly group-specific biomass has been obtained. The maximum productivity at all stages of the batch cultivation of N. meningitidis strains 125 and 133 in this medium does not differ from that at similar stages of cultivation in modified Cohen-Wheller semisynthetic medium. In the serotype antigen preparations obtained from N. meningitidis strain 125 grown in the above-mentioned liquid synthetic culture medium basic polypeptides with a molecular weight of 33000, 36000 and 41000 D have been detected. Their presence in N. meningitidis cells is linked with the growth phase of the population.

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