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Very large scale suspension cultures of mammalian cells

Pullen, K.F.; Johnson, M.D.; Phillips, A.W.; Ball, G.D.; Finter, N.B.

Developments in Biological Standardization 60: 175-177

1985

ISSN/ISBN: 0301-5149
PMID: 3899781
Document Number: 251025
Suspension cultures of mammalian cells can be used to produce virus vaccines, as a source of interferons, and as a host system for expressing inserted human genes. We will discuss the experience with this technology in our organisation over the past 18 years, during which we have progressively expanded our facilities for such work: we are now operating routinely with 8 000 litre fermenters. Information will be summarized in relation to the following topics: design of plant, medium and serum requirements, plant operation and product processing.

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