Signal requirements for growth and differentiation of activated murine B lymphocytes
Müller, W.; Kühn, R.; Goldmann, W.; Tesch, H.; Smith, F.I.; Radbruch, A.; Rajewsky, K.
Journal of Immunology 135(2): 1213-1219
1985
ISSN/ISBN: 0022-1767 PMID: 3874233 Document Number: 251018
Mouse B lymphocytes were stimulated at high cell concentrations with goat anti-IgM antibodies, which leads to the induction of B cell proliferation without the addition of any growth factors. After 48 h, blast cells were purified and cultured at low cell concentrations. Proliferation and differentiation of purified B lymphocyte blasts is then dependent on the addition of either mitogens (e.g., lipopolysaccharide) or certain lymphokines derived from activated T cells or macrophages. One such lymphokine was isolated from supernatants of various activated T cells and characterized by gel filtration as a material with an apparent MW, of 40,000-50,000, similar to BCGF (B cell growth factor) II. It supports the proliferation of the B cell blasts and induces their differentiation into plaque-forming cells. Lymphokines such as BCGF I, interleukin 2, and BCDF (B cell differentiation factor).gamma. could neither maintain growth nor induce differentiation of B lymphocytes preactivated by goat anti-IgM.