The murine IFN-gamma receptor does not transduce the activation signal to a murine promoter in human cells

Lembo, D.; Cofano, F.; Cavallo, G.; Landolfo, S.

Journal of Biological Regulators and Homeostatic Agents 6(1): 26-30

1992


ISSN/ISBN: 0393-974X
PMID: 1332429
Document Number: 397399
MuIFN-gamma receptor cDNA has been stably transfected in the human WISH cell line. A stable transfectant, denominated WISH-C2, expressed 46;000 receptors/cell whose affinity was similar to that observed on murine cells (Kd = 2.6 x 10(-9) M). When WISH-C2 cells were treated with Hu- or MuIFN-gamma, antiviral and antiproliferative activities were observed with HuIFN-gamma only. These findings suggest that other species-specific components associated to the binding site are required for the signal transduction. To overcome the complexity of measuring biological functions that very likely involve more than one regulatory and structural gene, we set up for the first time a system where a reporter gene driven by a murine promoter was used to directly evaluate the interaction of MuIFN-gamma receptor with an inducible promoter through the missing transducer factor.

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