Quantification of serum inorganic phosphorus, phosphatase, and urinary phosphate without preliminary treatment
Goodwin, J.F.
Clinical Chemistry 16(9): 776-780
1970
ISSN/ISBN: 0009-9147 PMID: 4321811 Document Number: 25076
The phosphomolybdate complex of inorganic phosphate formed in the presence of borate is reduced with ascorbic acid. The resulting suspension is made soluble with carbonate and the absorbance is measured at 720 rm. The method was applied directly to serum and dilute urine and was also used to estimate acid and alkaline phosphatase in serum. Citrate, oxalate, mannitol, F-and NO2-did not interfere.
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