Formation and physico-chemical characteristics of exopolysaccharides from various bacteria of the Bacillus genus
Pirog, T.P.; Slabospitskaia, A.T.; Votselko, S.K.; el Said, M.; Afonskaia, S.V.; Grinberg, T.A.
Mikrobiolohichnyi Zhurnal 47(6): 27-32
1985
ISSN/ISBN: 0201-8462 PMID: 3916972 Document Number: 250073
The intensity of exopolysaccharides (EPS) synthesis of the Bacillus polymyxa and Bac. oligonitrophilus cultures is established to be affected both by the form and concentration of the nitrogen source of nutrition. The studies in dynamics of the growth process and formation of EPS in Bac. oligonitrophilus permit establishing the culture growth stages corresponding to the maximal specific rate of the EPS formation and maximal concentration of EPS in the culture fluid, that makes it possible to determine the duration of the producer cultivation for the maximal yield of the product. The data of the EPS sedimentation analysis showed that Bac. polymyxa and Bac. oligonitrophilus are heterogenous. The mean molecular weight of the Bac. oligonitrophilus EPS is 150000. Bac. polymyxa synthesizes the complex of EPS with the molecular weight of components from 30000 to 100000. The acidic EPS cultures consist of glucose, galactose, mannose and fucose. IR-spectra of the studied EPS cultures evidence for similarity of the chemical composition of exopolysaccharides.