Tauroursodeoxycholate prevents biliary protein excretion induced by other bile salts in the rat
Kitani, K.; Ohta, M.; Kanai, S.
American Journal of Physiology 248(4 Pt 1): G407-G417
1985
ISSN/ISBN: 0002-9513 PMID: 2984943 Document Number: 247402
Biliary excretion of various proteins (5'-nucleotidase, alkaline phosphatase, lactate dehydrogenase and albumin) was investigated in pentobarbital sodium-anesthetized rats infused with different bile salts [taurocholate (TC), taurochenodeoxycholate (TCDC) and tauroursodeoxycholate (TUDC)]. A TCDC infusion at 0.4 .mu.mol .cntdot. min-1 .cntdot. 100 g body wt-1 caused much higher increases in the biliary excretion of these proteins compared with the respective values in rats that received an infusion of TC at a 3-fold higher rate (1.2 .mu.mol .cntdot. min-1 .cntdot. 100 g body wt-1). In contrast, a TUDC infusion at 1.8 .mu.mol .cntdot. min-1 .cntdot. 100 g body wt-1 showed the minimum effect on these protein leakages. A combined infusion of TCDC (0.4 .mu.mol .cntdot. min-1 .cntdot. 100 g-1) and TUDC (0.6 .mu.mol .cntdot. min-1 .cntdot. 100 g-1) resulted in drastic (8- to 20-fold) decreases in excretion of these enzymes and albumin compared with respective values in rats infused with TCDC alone. Similar preventive effects were observed with the addition of TUDC to the infusion of TC (1.2 .mu.mol .cntdot. min-1 .cntdot. 100 g-1). The hepatic cytotoxic effects of TC and TCDC can be prevented by the simultaneous infusion of TUDC in rats.