Field evaluation of a rapid immunochromatographic test 'Parachek-F' in a post-monsoon Plasmodium falciparum malaria outbreak in villages of South India: indigenous diagnostic test kit for P. falciparum

Ghosh, S.K.; Sathyanarayan, T.S.; Murugendrappa, M.V.; Subbarao, S.K.

Japanese Journal of Tropical Medicine and Hygiene 30(1): 7-13


Document Number: 246223
The diagnostic value of a rapid immunochromatographic test (Parachek-F) was evaluated under field condition in a post-monsoon outbreak of malaria, mostly of Plasmodium falciparum, in 2 villages of Raichur, Karnataka, India, in November 1999. The test was based on dipstick P. falciparum histidine rich protein-2 (PfHRP-2) antigen capture assay. Of the 232 subjects (128 were males and 104 were females; aged 3 months-80 years) enrolled in the study, 158 (68.1%) were malaria positive. Of whom, 13 were infected with P. vivax, 140 with P. falciparum and 5 had mixed infections. Of the 145 P. falciparum cases, 107 were with rings only, 32 with rings and gametocytes, and 6 with gametocytes only. The Parachek-F test detected PfHRP-2 antigen in 136 of P. falciparum patients and was non-reactive to P. vivax cases. Considering P. falciparum rings only, the test detected 99 of 107 cases, whereas in the group with gametocytes only, 4 of 6 cases were detected. All of the 32 cases having concomitant rings and gametocytes showed positive to both microscopy (standard) PfHRP-2 antigen. Thus the sensitivity of the Parachek-F was 93.1%, specificity was 98.8%, positive predictive value was 99.2%, negative predictive value was 89.6% and test efficacy was 95.2%. In cases with low parasitaemia (<100 parasites/ micro l blood), Parachek-F failed to detect infection in 10 of 25 patients, which showed a sensitivity of 60%. It is concluded that Parachek-F is suitable for field condition.

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