Chromosome mapping by in situ hybridization of cloned human DNA sequences
Patracchini, P.; Calzolari, E.; Aiello, V.; Contiero, M.R.; Del Senno, L.; Gambari, R.; Marchetti, G.; Bernardi, F.
Bollettino della Societa Italiana di Biologia Sperimentale 61(4): 475-482
1985
ISSN/ISBN: 0037-8771 PMID: 4041252 Document Number: 243433
"In situ" hybridization allows the chromosomal localization of cloned DNA sequences. In the present paper we report "in situ" hybridization experiments to localize: (A) 7SL RNA genes, (B) the sequences homologous to a clone (29 .times. 3) from a .times. chromosome library and (C) the human thyroglobulin gene. (A) "In situ" hybridization with the SAU 3A fragment of a 7SL RNA gene shows extensive labeling in different chromosomes, indicating that the 7SL genes (approximately 1000 copies) are spread in the human genome. (B) The DNA sequences 29 .times. 3 (approximately 150 copies in the genome) significatively hybridize with the chromosomal sites: X q22-25, 4 p15-14, 5 q31-33 e 16 q11-13. (C) the localization of the autoradiographic grains, obtained with the 2 Kilobases thyroglobulin genomic probe, suggests a sub-regional assignment of the human thyroglobulin gene to 8 q23-24.