Metabolism of 2-methylnaphthalene by rat and rainbow trout hepatic microsomes and purified cytochromes P-450
Melancon, M.J.; Williams, D.E.; Buhler, D.R.; Lech, J.J.
Drug Metabolism and Disposition the Biological Fate of Chemicals 13(5): 542-547
1985
ISSN/ISBN: 0090-9556 PMID: 2865100 Document Number: 242987
The metabolism of 14C-2-methylnaphthalene by hepatic microsomes and purified cytochromes P-450 from rat and rainbow trout was studied using HPLC and radioactivity counting. The effects of pretreatment with beta-naphthoflavone and phenobarbital on the formation of three dihydrodiol metabolites and 2-hydroxymethylnaphthalene by microsomes and purified cytochromes P-450 were compared. The metabolism observed with microsomes could not adequately be accounted for by the combined metabolism observed with the purified cytochromes P-450. Differences in the microsome suspension buffers used when preparing microsomes for cytochrome purification as compared to preparing microsomes for direct use appeared to be a significant factor in these differences.