In vitro synthesis of interleukin 2 (IL-2) by T lymphocytes extracted from a lymphoepithelial thymoma. Purification and biochemical characterization
Ponzoni, M.; Fenice, M.; Montaldo, P.; Tonini, G.P.; Cornaglia-Ferraris, P.
Bollettino dell'Istituto Sieroterapico Milanese 64(1): 12-24
1985
ISSN/ISBN: 0021-2547 PMID: 3873951 Document Number: 241985
T (E+) lymphocytes purified from lymphoepithelial thymoma surgically removed from an asymptomatic 12 yrs old girl were cultured in presence of PHA-P; culture supernatant contained a T cell growth factor (IL-2) biochemically different from that purifiable from supernatants of normal T cells. Differences in molecular weight, isoelectric point and ion exchange elution pattern would allow to compare this abnormal IL-2, (named Thy-IL-2 from Thymus) to that already characterized from the HUT 102 T cell line (L-TCGF peak II). A comparison of biochemical properties of Thy-IL-2 and normal IL-2 (N-IL-2) is also presented; the biological significance of such differences are discussed.