Restoration and stimulation of the in vitro immune response of B cells to sheep erythrocytes by interleukins and muramyl dipeptide (MDP)
Souvannavong, V.; Adam, A.
Biochemical and Biophysical Research Communications 125(1): 431-439
1984
ISSN/ISBN: 0006-291X PMID: 6334520 Document Number: 239721
MDP [N-acetylmuramyl-L-alanyl-D-isoglutamine], a synthetic muramyl dipeptide, is capable of increasing the primary in vitro antibody response to sheep erythrocytes of purified B cells restored with a monokine and helper T cell factors, including interleukin 2 and the late-acting T-cell replacing factor (TRF). The possibility that the adjuvanticity of MDP could be due to the elaboration of interleukin 1, caused by its effect on macrophages, was excluded. A kinetic study showed that the effect of MDP was greater when added later, concomitantly with or 1 day after the helper T cell factors. MDP apparently acts directly on B cells, in a late stage of their differentiation to antibody-producing cells.