Purification of neuraminidase from influenza virus on an immunosorbent
Iakubov, L.A.; Savich, I.M.; Beklemishev, A.B.
Biokhimiia 49(10): 1588-1593
1984
ISSN/ISBN: 0320-9725 PMID: 6440594 Document Number: 238172
A procedure for isolation of neuraminidase from influenza virus using the nonionic detergent Triton X-100 was developed. To achieve further purification, the protein mixture was passed through a Sepharose column packed with immobilized antibodies against hemagglutinin. The neuraminidase preparation thus obtained fully retained its enzymatic and antigenic properties and during electrophoretic separation under denaturating conditions gave 1 protein band.